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biotinylated rat anti mouse f4 80  (Bio-Rad)


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    Bio-Rad biotinylated rat anti mouse f4 80
    Biotinylated Rat Anti Mouse F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 5807 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rat anti mouse f4 80/product/Bio-Rad
    Average 96 stars, based on 5807 article reviews
    biotinylated rat anti mouse f4 80 - by Bioz Stars, 2026-03
    96/100 stars

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    ( A and B ) Schematic and weight change of Con and Adip-KO mice with 21 weeks of HFD followed by 13 weeks of chow diet ( n = 5, 5, respectively). ( C ) Body composition analysis of female Con and Adip-KO mice before and after diet change from HFD to chow diet ( n = 4, 4, respectively). ( D and E ) q-PCR analysis of inflammatory gene ( D ) and components of inflammasome ( E ) in VAT macrophages <t>(F4/80</t> + ) in obese mice switched to chow diet ( n = 4, 4, respectively). ( F ) Inflammasome activation after pretreatment of SPARC protein for 24 hours following ATP (5mM) treatment with or without LPS (1 μg/mL) measured by caspase-1 Western blot analysis in cell lysate (lower) and supernatant (upper). Error bars represent the mean ± SEM. 2-tailed unpaired t tests were performed for statistical analysis. * P < 0.05; ** P < 0.01; *** P < 0.001.
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    ( A and B ) Schematic and weight change of Con and Adip-KO mice with 21 weeks of HFD followed by 13 weeks of chow diet ( n = 5, 5, respectively). ( C ) Body composition analysis of female Con and Adip-KO mice before and after diet change from HFD to chow diet ( n = 4, 4, respectively). ( D and E ) q-PCR analysis of inflammatory gene ( D ) and components of inflammasome ( E ) in VAT macrophages (F4/80 + ) in obese mice switched to chow diet ( n = 4, 4, respectively). ( F ) Inflammasome activation after pretreatment of SPARC protein for 24 hours following ATP (5mM) treatment with or without LPS (1 μg/mL) measured by caspase-1 Western blot analysis in cell lysate (lower) and supernatant (upper). Error bars represent the mean ± SEM. 2-tailed unpaired t tests were performed for statistical analysis. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Journal: The Journal of Clinical Investigation

    Article Title: Reduction of SPARC protects mice against NLRP3 inflammasome activation and obesity

    doi: 10.1172/JCI169173

    Figure Lengend Snippet: ( A and B ) Schematic and weight change of Con and Adip-KO mice with 21 weeks of HFD followed by 13 weeks of chow diet ( n = 5, 5, respectively). ( C ) Body composition analysis of female Con and Adip-KO mice before and after diet change from HFD to chow diet ( n = 4, 4, respectively). ( D and E ) q-PCR analysis of inflammatory gene ( D ) and components of inflammasome ( E ) in VAT macrophages (F4/80 + ) in obese mice switched to chow diet ( n = 4, 4, respectively). ( F ) Inflammasome activation after pretreatment of SPARC protein for 24 hours following ATP (5mM) treatment with or without LPS (1 μg/mL) measured by caspase-1 Western blot analysis in cell lysate (lower) and supernatant (upper). Error bars represent the mean ± SEM. 2-tailed unpaired t tests were performed for statistical analysis. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Article Snippet: For positive selection of macrophages, SVF from VAT was incubated with biotinylated anti-mouse F4/80 antibody (eBioscience, 13-4801-85) in isolation buffer (PBS, 0.1% BSA, 2mM EDTA, pH 7.4).

    Techniques: Activation Assay, Western Blot